Once the software is installed, you should "unmount" the file so it’s no longer taking up a virtual drive slot.
Use a tool like Rufus (Windows) or Etcher (Mac/Linux). These apps take the ISO file and "burn" it onto a USB flash drive. Use Iso To Install Software
On modern computers (Windows 8/10/11 and macOS), you don’t need extra software to "open" an ISO. You simply it, which makes your computer treat the file like a disc you just plugged in. Once the software is installed, you should "unmount"