Protocols | Imaging Flow Cytometry: Methods And

: Cells must be in a single-cell suspension to avoid clogging the thin fluidic channels used for imaging.

IFC provides a "visual verification" for every data point, allowing researchers to see exactly what their gated populations look like. Imaging Flow Cytometry: Methods and Protocols

: Systems like the ImageStream use Charge-Coupled Device (CCD) cameras with Time Delay Integration (TDI) to track cells in flow, preventing motion blur and enabling the detection of dim markers. : Cells must be in a single-cell suspension