G9048.mp4 -

: Observations of "leader cells" responding to GLI2 inhibition.

: Injured epithelial cells release Sonic Hedgehog (SHH) ligands. g9048.mp4

: The resulting stiffened tissue triggers non-canonical activation of the transcription factor GLI2 in epithelial cells. : Observations of "leader cells" responding to GLI2

: Time-lapse footage of cancer cells moving across substrates of varying stiffness. g9048.mp4

: This signaling activates fibroblasts, which increase tissue stiffness by producing collagen.

: This "chemo-mechanical" loop promotes cell proliferation, migration, and the transition of normal cells into invasive cancer cells. Potential Video Content

: Visual evidence of cell mobility and invasiveness.

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