Protocols | Crispr: Methods And

: Often results in small insertions or deletions (indels), effectively knocking out a gene [5, 33].

: Create a guide RNA complementary to the target sequence [26]. CRISPR: Methods and Protocols

The standard CRISPR-Cas9 system consists of two primary parts: : Often results in small insertions or deletions

: Directly injecting reagents into embryos or cells [15, 31]. effectively knocking out a gene [5